Soil organic matter has great importance in chemical, physical and biological processes in soil-plant system. This study aimed to evaluate changes in carbon humic fractions of soil organic matter under different vegetation coverings in Cerrado in Tocantins, Brazil. The work was developed in Eucalyptus sp., Pasture, Agriculture and Cerrado sensu stricto areas. Soil samples were collected in dry period, October, 2018, in trenches 70 x 70 cm at depths 0-10, 10-20, 20-30, 30-40 and 40-50 cm, with six replications. The vegetation cover in Eucalyptus sp. area showed higher levels of total carbon in soil humic fraction. Respectively fulvic acid, humic acid and humin in Eucalyptus sp. area had stocks of 22.09; 2.71 and 20.01% higher than native forest, 32.04; 2.57 and 35.59% higher than pasture and 10.59; 19.19 and 7.61% higher than the agriculture area. Among the different areas evaluated, the soil of Eucalyptus sp. has great potential to increase carbon storage in soil humic fractions.