This study analyzed the use of the water balance, precipitation, temperature and NDVI (Normalized Difference Vegetation Index) to determine the optimum moment for evaluating infestation by the nematode Meloidogyne exigua in a rubber tree plantation by remote sensing. Nineteen sampling points were defined, divided into three areas, with high and low defoliation degrees, on a property in the Triângulo Mineiro region, where soil and root samples were collected for nematological analysis. Monthly digital images (OLI - Landsat 8 sensor) were obtained from August 2015 to August 2016. Rainfall, temperature and water balance were analyzed. The latter had no correlation with the indices; in contrast, temperature and precipitation correlated directly with NDVI. The best months for nematode soil infestation analysis were December, January and February, while for root infection it was August.