To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell
line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by
electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were
respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was
detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome
DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1
were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot.
EMSA demonstrated specific binding of the E2F decoy to E2F transcription factor.The PC-3M cell growth was
inhibited after transfection. The apoptotic rate was 26.35% and DNA ladder could be observed after
transfection.The expression of C-myc and CyclinD1 were inhibited. All these results indicated that E2F decoy DNA
induced apoptosis of androgen-independent prostate cancer cell lines PC-3M and inhibited cell proliferation via
inhibiting expression of C-myc and CyclinD1.
