Changes in Growth and Photosynthetic Capacity of Cucumber Seedlings in Response to Nitrate Stress

Ano: 2009 | Volume: 21 | Número: 4

Autores: Xiaoyu Yang, Xiufeng Wang, Min Wei, Shoko Hikosaka, Eiji Goto

Palavras-chave: chlorophyll a fluorescence, hill reaction, photosynthetic pigment content, photosynthetic rate

Resumo Inglês:

The effects of three nitrate levels - 14(CK), 56(T-1), and 140 mmol L-1 (T-2) - on growth and photosynthetic capacity of cucumber
(Cucumis sativus L. cv. Xintaimici) seedlings grown in hydroponic culture were investigated. The results showed that at 12 d after
treatment plant height, stem diameter, leaf area, and leaf number of cucumber seedlings were stimulated by 56 mmol L-1 nitrate, whereas
were inhibited significantly by 140 mmol L-1 nitrate compared with CK. Short-term stimulation in photosynthetic rate occurred under T-1
treatment, and then recovered to the level of CK. Photosynthetic rate of T-2 seedlings significantly decreased over treatment course with
respect to CK. Photosynthetic pigment content of T-1 and T-2 increased during the first 2 d, and gradually recovered to the level of CK
thereafter. Chlorophyll a/b and carotenoids/chlorophyll of T-1 had no significant difference from CK during treatment period. During the
first 4 d, there was no significant difference in chlorophyll a/b and carotenoids/chlorophyll between T-2 and CK. After 4 d, chlorophyll a/b
of T-2 increased gradually, whereas carotenoids/chlorophyll decreased. Actual PSII efficiency (ΦPSII) and photochemical quenching (qP)
of T-1 had no significant difference from CK, and non-photochemical quenching (qN) was a little higher than CK after 2 d. During the
first 2 d, there was little difference in ΦPSII and qP between T-2 and CK. After 2 d, both ΦPSII and qP of T-2 decreased to a great extend. A
significant increase in qN of T-2 occurred over treatment course. With respect to CK, Hill reaction activity of T-1 slightly decreased, and
T-2 treatment resulted in a significant decrease of Hill reaction activity. This evidence indicates that high-level nitrate stress may reduce
photosynthesis through its effects not only on stomatal conductance but on the photosynthetic apparatus.