In the face of the human impacts caused mainly after the Industrial Revolution, such as the burning of fossil fuels, deforestation and intensive soil preparation, there has been an increase in the emission of greenhouse gases (GHG). Among the forms of GHG mitigation, there are those carried out through agriculture, such as the adequate management of the soil and the removal of CO 2 by the plants through the consortium systems. Studies indicate that a viable alternative is the forest-livestock integration system (iLPF), which is composed of tree and forage species. Measurement of the concentration of atmospheric CO 2 ([CO 2]) in iLPF can indicate answers that will serve as a basis for the monitoring of this ecosystem in the Amazon region. Vertical profile measurements of [CO 2 ] were obtained in intensive campaigns during the less rainy periods of 2016, and more rainy and less rainy season of 2017, in the municipality of Terra Alta, PA. There was greater amplitude in the [CO 2 ] measured in the less rainy period of 2017 and higher daytime concentrations in relation to the other periods. Seasonality is a factor that influences [CO 2 ] in the iLPF system. The [CO 2 ] only begins to rise and accumulate below the canopy from 20:00 hours, when the atmosphere is more stable.