The Construction of a Short Gene by a Very Fast, Modified, and Simplified Gene Synthesis and the Analysis of Various Effects on This Synthesis
Brazilian Archives Of Biology And Technology
Endereço:
Rua Professor Algacyr Munhoz Mader 3775, CIC
Curitiba / PR
0
Site: http://www.scielo.br/babt
Telefone: (41) 3316-3012
ISSN: 15168913
Editor Chefe: Carlos Ricardo Soccol
Início Publicação: 30/11/1946
Periodicidade: Bimestral
Área de Estudo: Biologia geral
The Construction of a Short Gene by a Very Fast, Modified, and Simplified Gene Synthesis and the Analysis of Various Effects on This Synthesis
Ano: 2011 | Volume: 54 | Número: 1
Autores: Mohammadreza Hajjari, Behnaz Saffar
Autor Correspondente: Behnaz Saffar | [email protected]
Autor Correspondente: Behnaz Saffar | [email protected]
Palavras-chave: single step assembly pcr, efficiency, amplification primer, polymerase
Resumos Cadastrados
Resumo Inglês:
We have constructed a short gene with a single step assembly PCR without any additional amplification primers
with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions,
we have analyzed various effects on its efficiency. Eventually, we have been able to synthesize the gene in less than
40 minutes by Taq polymerase.