Este estudo teve como objetivo detectar a presença de búfalos soro reagentes para Brucella sp. e Leptospira spp. Foram utilizadas amostras de soro de 113 animais provenientes da Ilha do Marajó, abatidos em matadouro frigorífico na cidade de Belém-PA, as quais foram submetidas ao teste de soroaglutinação rápida em placa com Antígeno Acidificado Tamponado (AAT) e a prova 2-Mercaptoetanol (2-ME) para a pesquisa de anticorpos contra Brucella sp. e ao Teste de Aglutinação Microscópica (MAT) para pesquisa de aglutininas contra Leptospira spp. Das 113 amostras analisadas, 13,3% (15/113) foram reagentes para anticorpos de Brucella sp. no AAT e 6,1% (7/113) foram confirmada na prova de 2-ME. Para Leptospira spp., 35,4% (40/113) dos bubalinos foram reagentes com o sorovar Hardjo C.T.G., presente em 75% (30/40) das reações. Foram detectados anticorpos contra os agentes pesquisados, demostrando a necessidade de se fazer um controle sanitário mais eficiente no rebanho.
The present study aimed to detect the presence of buffalos serum reagents for Brucella sp. and Leptospira spp. Serum samples from 113 animals from Marajó Island, slaughtered at a slaughterhouse in the city of Belém, Pará, were used, which were submitted to the Rapid Agglutination Test with buffered, acidified plate antigen (BAPA) and 2-Mercaptoethanol ( 2-ME) for the detection of antibodies against Brucella sp. and the Microscopic Agglutination Test (MAT) for the detection of agglutinins against Leptospiraspp. From the 113 samples analyzed, 13.3% (15/113) were reagents for Brucella sp. antibodies in AAT and 6.1% (7/113) were confirmed in the 2-ME test. For Leptospira spp., 35.4% (40/113) of the buffalos were reactive with serovar Hardjo C.T.G., present in 75% (30/40) of the reactions. It was concluded that antibodies against the investigated agents were detected, demonstrating the need to make a more efficient sanitary control in the herd.
