The antifungal activity of Penicillium steckii (MF1) against pathogenic fungi of rose causing verticillium wilt was
evaluated under differential nutrient condition. A suitable and modified medium was obtained in which test fungus
exhibited enhanced antifungal activity and produced larger inhibition zone against the verticillium fungus. The
study carried out to modify the medium components at level of nitrogen, carbon source and their concentration on
agar plates. Addition of amino acids, metals and plant growth hormones did not show positive effect but glycerol
enhanced the antifungal activity very much.
