The potential of the white rot fungus, Coriolus versicolor ML04 to decolorize the widely used textile dye Blue BB
was tested by employing statistical optimization. Response surface methodology (RSM) involving a central
composite design (CCD) was applied to evaluate the interactive effects of four significant factors in different ranges
i.e., glucose (0.5 – 2.5 g/L), yeast extract (0.4 –1.2 g/L), dye concentration (100 – 500 ppm) and inoculum size (5 –
20 % v/v) to decolorize the Blue BB. The results demonstrated the effectiveness of the statistical experimental design
and the ability of C. versicolor ML04 for maximum dye decolorization (>96%) at the optimum conditions of the
significant factors.
