Extraction and determination of ascorbate and dehydroascorbate from apoplastic fluid of stem of rooted and non-rooted cuttings in relation to the rhizogenesis

Ano: 2010 | Volume: 22 | Número: 2

Autores: Katia Christina ZUFFELLATO-RIBAS, Stefano Morini, Piero Picciarelli, Francesco Mignolli

Palavras-chave: olive tree, peach rootstock, plum rootstock, redox potential, rooting

Resumo Inglês:

Starting from semihardwood branches of peach and plum rootstocks (Prunus cerasifera E.) cv. MRS2/5, plum rootstocks (Prunus
cerasifera × P. salicina) × (P. cerasifera × P. persica) cv. Ishtarà and olive tree (Olea europaea L.) cv. Frantoio, cuttings were
collected and treated with 0 mg.L-1 and 2500 mg.L-1 of indolebutyric acid (IBA) by immersion in the base of the cuttings. After 60
days of planting, the cultivars MRS 2/5, Ishtarà and Frantoio presented a significative difference with relation to the rooting induction
by application of the indolebutyric acid (27.5, 47.5 and 41.25 %, respectively), inducing a higher number of roots per cutting without
at the same time significantly increasing the length of the roots themselves. The levels of ascorbate and dehydroascorbate in the
rooted and non-rooted cuttings of the three cultivars were determined. These parameters varied in function of the consistency of the
cuttings from each species, but showed a positive correlation between the increase of the rooting and the increase of the ascorbate
in the reduced form.