Tropical forests include remnants that should be characterized and monitored for long-term conservation. With this in mind, we performed a survey of the floristic composition and structure of the Mata Santa Elisa (Campinas, SP), and compared it to other fragments of semi-deciduous seasonal forest in the municipality. In Mata Santa Elisa, 506 living arboreal specimens were found, belonging to 100 species, 75 genera and 32 families. At the time of this work, the fragment was well-preserved and contained exclusive species and those of economic interest or potential, highlighting its importance in the region.