Genetic Variability among the Wild Boars (Sus Scrofa Scrofa), Crossbred Animals and Pigs Using Microsatellite Markers (STRs)

Ano: 2011 | Volume: 54 | Número: 2

Autores: Paula Viana Correa da Silva, Jeffrey Frederico Lui, Guilherme de Oliveira Band, Luciana Correia de Almeida Regitano, Selma de Fátima Grossi, Bruna Pena Sollero, Cleujosí da Silva Nunes

Palavras-chave: molecular genetics, wild boars, microsatellites, fragments, amplification

Resumo Inglês:

The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using
microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus
scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra
populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (FIS) within each
population and inter population variability FST, testing relationship among five genetic groups to establish the
genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871.
Generally, FIS was low, suggesting that the endogamy did not exist between the tested animals.