This study evaluated the effect of growth bacteria and nano iron fertilizer and ordinary iron fertilizer on the chlorophyll content and concentration of some nutrients. The experiment was conducted as 4 × 6 factorial in RCBD design with three replications in two years (2016-2017). Treatments included: use of plant growth-promoting bacteria in four levels (Non-inoculation, inoculation with Azotobacter crocococcus, Azospirillium methylpofrome, and Pseudomonas putida) and nano iron fertilizer in five levels (0.0 %, 0.5 %, 1.0 %, 1.5 %, and 2.0 %) and ordinary iron fertilizer on two levels (2.0 % and 0.0 %). Based on the results, the application of biofertilizer and Fe fertilizer had significant effects on all traits at 1.0 % or 5.0 % level. The results of the comparison of the mean of treatments showed that the highest chlorophyll a (0.806) was obtained by Azotobacter crocococcus + 0.5 % nano-Fe and the highest means of chlorophyll b and carotenoid with 0.275 mg g FW-1 and 0.224 (mg g FW-1) values, respectively, were observed by Pseudomonas putida + 0.5 % nano-Fe. The highest value of P (55.24), N (4.42) and Fe (84.43) were obtained by Pseudomonas putida + control Fe, Azotobacter crocococcus + 1 % nano-Fe and Pseudomonas putida + 1 % nano-Fe, respectively.
