Foram isoladas e caracterizadas bactérias presentes no lodo de esgoto
visando à realização de estudos futuros de interação desses organismos
com fi tonematóides. Os lodos utilizados são provenientes das estações de
tratamento de Franca e Barueri, no Estado de São Paulo. Foram obtidos
77 isolados do lodo de Franca e 143 do de Barueri. Desses isolados de
Franca testados, 77% foram Gram-positivos e 23% Gram-negativos.
Para os 143 isolados obtidos do lodo de esgoto de Barueri, 61% foram
Gram-positivos e o restante Gram-negativo. Os isolados Gram-positivos
podem, estar relacionados com espécies do gênero Bacillus dos quais
várias espécies têm grande importância como agentes de controle de
fitopatógenos, inclusive nematóides. Observou-se que 39% e 40%
dos microrganismos Gram positivos, isolados dos lodos coletados na
ETE de Franca e de Barueri, produziram pigmento fl uorescente em
meio King B, sendo indicativo de Pseudomonas fl uorescens. Dos 77
isolados de Franca, 13% apresentaram como caracterÃstica morfológica
bordo lobado, caracterÃstica de culturas de Bacillus. Comparando-os com os testes bioquÃmicos, concluiu-se que 80% deles foram Grampositivos,
caracterÃstica de espécies de Bacillus. Dos isolados, 10%
apresentaram bordo inteiro, colônia brilhante e arredondada, as quais são
caracterÃsticas de Pseudomonas. Desses, 37% foram Gram-negativos,
sendo que 67% desses isolados eram também produtores de pigmento
fluorescente em meio King B, caracterÃsticas da P. fluorescens. Dos 143
isolados de Barueri, 17% apresentaram bordo lobado como caracterÃstica
morfológica, podendo-se suspeitar de serem espécies de Bacillus e 5%
apresentaram as caracterÃsticas de espécies de Pseudomonas. Para os
isolados do lodo de Barueri, na comparação com os testes bioquÃmicos
constatou-se que 64% dos isolados foram Gram-positivo. Dos isolados
com caracterÃsticas de Pseudomonas, 17% apresentaram-se como Gramnegativas
e desses, 17% mostraram-se como produtores de pigmento
fluorescente em meio King B, que são caracterÃsticas de espécies de
Pseudomonas do grupo fl uorescente.
This work aimed at isolating and characterizing bacteria presented in
sewage sludge with the objective of future studies for the interaction of
these organisms with nematodes. For this study, it was used samples of
the sewage sludge originated from the treatment stations of the cities of
Franca and Barueri, in the state of São Paulo. It was obtained 77 samples
of isolated sewage sludge from Franca and 143 from Barueri. From the
Franca samples, 77% was Gram-positive and 23% was Gram-negative
while from the Barueri samples, 61% was Gram-positive and the rest was
Gram-negative. The isolated Gram-positive can be related to the species
of the genre Bacillus, whose species have great importance as agents of
the phytopathogens control, including nematodes. It was observed that
39% and 40% of the Gram-positive microorganisms isolated from the
collected sewage sludge in ETE from Franca and from Barueri produced
fluorescent pigments in King B medium, as indicative of Pseudomonas
fluorescent presence. By examining the 77 samples of sewage sludge
from Franca, 13% presented as morphologic characteristic, the margin
of lobes, characteristic of the Bacillus culture. When compared them to
the biochemistry tests, it was concluded that 80% was Gram-positive,
a characteristic of the Bacillus species. By isolating the samples of
sewage sludge from Franca, 10% presented whole margin, fl uorescent
and round colony, characteristics of the Pseudomonas while 37% was Gram-negative, considering that 67% of this isolated sewage sludge
produced fl uorescent pigments in King B medium, characteristics of
Pseudomonas fl uorescence. By examining the 143 samples of sewage
sludge from Barueri, 17% presented the margin of lobes, as a morphologic
characteristic, suggesting that they belonged to the Bacillus species and
5% presented the characteristic of the Pseudomonas species. By isolating
the samples of sewage sludge from Barueri, it was found out, during the
comparison with the biochemistry tests, that 64% was Gram-positive. From
the isolated sewage sludge that presented the Pseudomonas characteristics,
17% indicated to be Gram-negative while 17% demonstrated to be
producers of fl uorescent pigments in King B medium, characteristics of
the Pseudomonas species from the fl uorescent group.
