NESFATIN-1/NUCLEOBINDIN-2 AND VISFATIN GENES RESPONSES TO 8 WEEKS OF TREADMILL RUNNING WITH OR WITHOUT PISTACHIO ATLANTICA LIQUID EXTRACTION IN FEMALE RAT TISSUE

Ano: 2012 | Volume: 6 | Número: 1

Autores: Saleh Rahmati-Ahmadabad, Abbass Ghanbari-Niaki, Navabeh Zare-Kookandeh, Zarbakht Ansari-Pirsarae

Palavras-chave: Aerobic training; Orexigenic peptide; Physical stress.

Resumo Inglês:

RAHMATI-AHMADABAD, S. ; GHANBARI-NIAKI, A .; ZARE-KOOKANDEH, N. ; ANSARI-PIRSARAE, Z.
Nesfatin-1/nucleobindin-2 and visfatin genes responses to 8 weeks of treadmill running with or without
pistachio atlantica liquid extraction in female rat tissue. Brazilian Journal of Biomotricity. v. 6, n. 1, p. 43-52,
2012. Nesfatin-1 is a protein derived from a precursor molecule of the nucleobindin-2 gene that effect on
food composition and inhibition of the orexigenic effect of orectic peptide such as visfatin (adipokine, was
revealed to be associated with obesity and to have insulin mimetic effect). It has been reported that several
factors (nutrients, fasting, physical stress) could effect on proteins and peptides gene expression. Pistacia
atlantica (Baneh) are plants of Anacardiaceae family that rich in unsaturated fatty acids and has antioxidative compounds that reduces free radicals. The aim of this study was to investigate the effects of eight
weeks of aerobic training with and without extract of Pistacia atlantica (6 weeks, 1 mg per 1 g body weight)
on nesfatin-1/nucleobindin-2 and visfatin genes of (intestine tissue) female rats. In this study twenty wistar
rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned into Saline-control
(SC), Saline-training (ST), and Baneh-control (BC), and Baneh-training (BT). Training groups was given
exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day and 5 days/week for eight
weeks. Animals were fed as oral, with Baneh extraction and Saline for four weeks. Nesfatin-1 and visfatin
gene expression was detected by Real-time PCR method. Results demonstrated that the exercise training
effect on Nesfatin-1/NUCB2 and visfatin expression but weren’t significant. This presented study showed a trend for exercise-induced increase and decrease in intestine expression of nesfatin-1 and visfatin mRNA,
respectively. Baneh extraction significantly reduces (P<0.001) and increases (P<0.028) nesfatin-1/NUCB2
and visfatin expression, respectively.