Due to its biological nitrogen fixation capacity, soybean cultivation has shown higher yields with the inoculation of Rhizobium sp. bacteria. However, the inoculation process can be influenced by several factors, including the fertilization performed. The aim of this research was to evaluate the influence of different fertilizations on nodulation and development of soybean plants. For this, the organic fertilizations, based on cattle manure and poultry litter, chemical fertilization and a control treatment were tested, with or without Bradyrhizobium japonicum inoculation. It was evaluated the plant height, leaf number, plant dry matter and the number and dry matter of nodules. The treatments with cattle manure showed the best results in all variables, with or without seed inoculation. Cattle manure is an excellent choice for soybean fertilization, since it allows a greater plant development and a higher number of nodules contributing to a higher yield at the end of the crop cycle.