Production and Characterization of Exopolysaccharides (EPS) from Biofilm Forming Marine Bacterium

Ano: 2011 | Volume: 54 | Número: 2

Autores: Muthusamy Ashok kumar, Kanapathi Thangavel Kasirajan Anandapandian, Karuppiah Parthiban

Palavras-chave: biofilm, marine bacteria, eps, sem, tlc and ftir

Resumo Inglês:

Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC
pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass
slides from the device after a month period of time. The efficiency of EPS production was checked for all the
isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied.
The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer
chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong
absorption band of –OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.