The aim of this work was to study the herbicidal potential of Cell free culture filtrate of Colletotrichum dematium
FGCC#20 against Parthenium by employing different bioassays i.e. shoot-cut, seedling, detached leaf and seed
germination. On solvent extraction of the Cell free culture filtrate, Ethyl acetate extracted fraction showed the
presence of phytotoxic moiety.