The aim of this work was to study the production of the bioinsecticide using the aerobic cultivation. Two-stage
cultivation process was used: aerobic vegetative growth and end-phase of sporulation (with aeration and without
aeration). The cuts of working volume evaluated were 20, 40, 60 and 80 %. The highest value of spore
concentration (2.17x109 CFU/mL) and spore productivity (7.2x107 CFU/mL.h) were observed using SP60. The
lethal concentration (CL50) obtained through the aeration process and non-aeration process were 0.21 and 0.05
mg/L, respectively. The specific potential of the bioinsecticide produced without aeration (0.025 mg/10-12 CFU) was
two times higher than that obtained with aeration (0.05 mg/10-12 CFU).
