The present work aimed to propose a basic operational model for the induction of in vitro germination of trees through plant tissue cultivation techniques. Fruits of 15 tree species, ten woody trees (Couroupita guianensis Aubl., Tabebuia heptaphylla (Vell.) Toledo, Tabebuia impetiginosa (Mart. ex DC.) Standl., Tabebuia roseo-alba (Ridl.) Sandwith, Vochysia haenkeana (Spreng.) Mart., Vitex montevidensis Cham., Copaifera coriacea Mart., Spondias tuberosa Arruda, Shinus terebinthifolia Raddi, and Talisia esculenta (A. St.-Hil.) Radlk.) and five palm trees (Syagrus coronate (Mart.) Becc., Attalea oleifera Barb. Rodr., Elaeis guineensis Jacq., Colubrina glandulosa Perk., and Astrocaryum vulgare Mart.), were collected at different locations in the state of Pernambuco, Brazil. For in vitro germination, we used two different protocols, one designed for palm trees and one designed for woody trees. We evaluated the parameters microbial contamination, survival, in vitro establishment, germination percentage and percentage of seeds converted to plants. The results demonstrated that the set of methodologies proposed as basic protocol for the in vitrointroduction was able to achieve satisfactory results for 13 of the 15 tested species. Thus, the protocol proposed here has a high potential for use in the redemption of seeds through in vitro plant tissue culture. The described technique is a useful tool for the propagation of trees used in reforestation programs.
