In Vitro Multiplication of ‘Flordaguard’ Rootstock: Cytokinin Source and Concentration Effects, Explants Orientation and Period of Permanence in the Culture Medium
Brazilian Archives Of Biology And Technology
In Vitro Multiplication of ‘Flordaguard’ Rootstock: Cytokinin Source and Concentration Effects, Explants Orientation and Period of Permanence in the Culture Medium
Autor Correspondente: Elizete Beatriz Radmann | [email protected]
Palavras-chave: micropropagation, cytokinin, explant, prunus
Resumos Cadastrados
Resumo Inglês:
This work aimed at studying the effects of different sources and cytokinin concentrations, as well as explants
orientation and time of their permanence in shoots induction medium to obtain high multiplication rate. Four
experiments were carried out to evaluate the sprout percentage mean, shoots number per explant, elongated shoots
percentage and shoots length. The treatments with 2iP and zeatin did not promote in vitro plant multiplication.
When different BAP concentrations were tested, an increase in sprout percentage was obtained using BAP
concentration up to 3.66 mg L-1 (80%), and for shoots number per explant a linear behavior was observed, showing
a mean of four shoots per explant with 4.0 mg L-1 BAP. For the ´Flordaguard´ rootstocks, the explants orientation
did not alter the potential in vitro multiplication and the shoots growth. However, the explants permanence in the
shoots induction medium for 40 days provided the best results for shoots growth.