In this study, BevMab was conjugated with the bifunctional chelating agent [diethylenetriamine pentaacetic acid
(DTPA)] and the product (BevMab-DTPA) was labeled with 99mTc using stannous chloride reducing method. The
quality control studies of radiolabeled compound (99mTc-BevMab-DTPA) were done with Thin Layer Radio
Chromatography (TLRC) and High Performance Liquid Radio Chromatography (HPLRC) methods (% 95 ≤) to
confirm the labeling efficiency. High radiochemical yield [98.07 % ± 2.17 (n = 13)] was obtained by TLRC method.
Biodistribution studies of 99mTc labeled BevMab-DTPA was run on healthy female and male Albino Wistar rats. The
distribution figures demonstrated that the radiolabeled compound was eliminated through the kidneys and
accumulated in urinary bladder. The values of the BevMab-DTPA uptakes were similar in heart, blood, liver and
spleen in both sexes.
